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Experiment ID	GSE8380
Experiment Title	Rice Gene Network Inferred from Expression Profiling of Plants Overexpressing OsWRKY13
Number of Samples	9
Organism(s)	Oryza sativa
Summary	Accumulating information indicates that plant disease resistance signaling pathway frequently interact with other pathways regulating developmental processes or abiotic stress responses. However, the molecular mechanisms of these types of crosstalk remain poorly understood in most cases. Here we report that OsWRKY13, an activator of rice resistance to both bacterial and fungal pathogens, functions as a convergent point of the crosstalk between pathogen-induced salicylate-dependent defense pathway and at least five other physiological pathways. Genome-wide analysis of the expression profiling of OsWRKY13-overexpressing lines showed that OsWRKY13 directly or indirectly regulates the expression of more than 500 genes, which are potentially involved in different physiological processes according to the classification of Gene Ontology database. Comparing the expression patterns of genes functioning in known pathways or cellular processes upon pathogen infection and the phenotypes between OsWRKY13-overexpressing and susceptible wild-type plants, we find that OsWRKY13 is also regulator of other physiological processes during pathogen infection. OsWRKY13-involved disease resistance pathway synergistically interacts with glutathione/glutaredoxin system and flavonoid biosynthesis pathway via OsWRKY13 to monitor redox homeostasis and may enhance the biosynthesis of antimicrobial flavonoid phytoalexins. Meanwhile, OsWRKY13-invloved disease resistance pathway antagonistically interacts with SNAC1-mediated abiotic-stress defense pathway, JA signaling pathway, and terpenoid metabolism pathway via OsWRKY13 to suppress salt and cold defense responses as well as may retard rice growth and development. Keywords: abiotic stress, bacterial blight, microarray, Oryza sativa, signal transduction, WRKY transcription factor
Overall design	To determine the genes directly or indirectly regulated by OsWRKY13 genome-widely, we performed the microarray analysis using the Affymetrix Genechip Rice Genome Array containing total 57381 probe sets. Two independent OsWRKY13-overexpressing rice lines, D11UM 1-1 and D11UM 7-2 (Qiu et al., 2007), and wild-type Mudanjiang 8, which was susceptible to Xoo and M. grisea, were used for the analyses.The experiment was repeated three times.
Contributor(s)	Qiu D,Xiao J,Xie W,Liu H,Li X,Xiong L,Wang S
Citation(s)

Submission date	5-Jul-07
Contact name	Deyun Qiu
E-mail(s)	qiudeyun@gmail.com
Phone	86-27-87281812
Fax	86-27-87287092
URL
Organization name	Huazhong Agricultural University
Department	College of Life Science and Technology
Lab	National Center of Plant Gene Research (Wuhan)
Street address	Luoshi Road
City	Wuhan
State/province	Hubei
ZIP/Postal code	430070
Country	China

	ID	Platform	Sample Name
1.	GSM207558	Affymetrix	Mudanjiang8 OsWRKY13 Overexpressing D11UM1-1 rep1
2.	GSM207559	Affymetrix	Mudanjiang8 OsWRKY13 Overexpressing D11UM1-1 rep2
3.	GSM207560	Affymetrix	Mudanjiang8 OsWRKY13 Overexpressing D11UM1-1 rep3
4.	GSM207562	Affymetrix	Mudanjiang8 OsWRKY13 Overexpressing D11UM7-2 rep1
5.	GSM207563	Affymetrix	Mudanjiang8 OsWRKY13 Overexpressing D11UM7-2 rep2
6.	GSM207564	Affymetrix	Mudanjiang8 OsWRKY13 Overexpressing D11UM7-2 rep3
7.	GSM207565	Affymetrix	Mudanjiang8 wild type control rep1
8.	GSM207566	Affymetrix	Mudanjiang8 wild type control rep2
9.	GSM207567	Affymetrix	Mudanjiang8 wild type control rep3