| Experiment ID |
GSE7197 |
| Experiment Title |
Down-regulation of OsSRT1 induces DNA fragmentation and cell death in rice
|
| Number of Samples |
6 |
| Organism(s) |
Oryza sativa |
| Summary |
In order to study the physiological function of OsSRT1, a 412 bp segment of the 3a€?-untranslated region of OsSRT1, which was not conserved with OsSRT2, was inserted in inverted repeats to build a construct for RNA interference (RNAi). The construct was used to transform an indica rice variety (Minghui63).To study whether the down-regulation of OsSRT1 affected gene expression, we compared the transcripts of the RNAi to the wild type plants by microarray analysis (Affymetrix). RNAs were isolated from young leaves of 11 day-old plants (before appearance of lesions in the RNAi plants). Affymetrix GeneChip?? Rice Genome Array were performed. Data was analyzed with SAM excel add-in and in-house perl scripts.Analysis of data from three biological repeats revealed that 521 genes are up-regulated, and 213 genes are down-regulated (with q a€¡° value at 5%).
Keywords: expression analysis
|
| Overall design |
In order to study the physiological function of OsSRT1, a 412 bp segment of the 3a€?-untranslated region of OsSRT1, which was not conserved with OsSRT2, was inserted in inverted repeats to build a construct for RNA interference (RNAi). The construct was used to transform an indica rice variety (Minghui63).To study whether the down-regulation of OsSRT1 affected gene expression, we compared the transcripts of the RNAi to the wild type plants by microarray analysis (Affymetrix). RNAs were isolated from young leaves of 11 day-old plants (before appearance of lesions in the RNAi plants). Affymetrix GeneChip?? Rice Genome Array were performed. Data was analyzed with SAM excel add-in and in-house perl scripts.Analysis of data from three biological repeats revealed that 521 genes are up-regulated, and 213 genes are down-regulated (with q a€¡° value at 5%). |
| Contributor(s) |
Huang L,Sun Q,Qin F,Li C,Zhao Y,Zhou D
|
| Citation(s) |
17468215 |
| | |
| Submission date |
6-Mar-07 |
| Contact name |
Qianwen Sun |
| E-mail(s) |
sunqianwen@webmail.hzau.edu.cn |
| Phone |
862787281800 |
| Fax |
|
| URL |
http://redb.ncpgr.cn/ |
| Organization name |
Huazhong Agriculture University |
| Department |
National Key Laboratory of Crop Genetic Improvement |
| Lab |
Gene Center |
| Street address |
Luoshi Road |
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430070 |
| Country |
China |
