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Experiment ID GSE6901
Experiment Title Expression data for stress treatment in rice seedlings
Number of Samples 12
Organism(s) Oryza sativa
Summary Drought, salinity and sub-optimal temperatures are stresses that cause adverse effects on the growth of plants and the productivity of crops. In this study, we have analyzed the expression profiles of rice genes under control and abiotic stress conditions using microarray technology to identify the genes differentially expressed during various abiotic stresses.
Keywords: Stress treatment

Overall design Seven-day-old light-grown rice seedlings grown under controlled conditions and those subjected to various abiotic stress conditions were used for RNA extraction and hybridization on Affymetrix microarrays. Three biological replicates of each sample were used for microarray analysis. For salt treatment (SS), the rice seedlings were transferred to a beaker containing 200 mM NaCl solution for 3 h. For desiccation (DS), rice seedlings were dried for 3 h between folds of tissue paper at 28?¡À1 degree C, in a culture room. For cold treatment (CS), the seedlings were kept at 4?¡À1 degree C for 3 h. The seedlings kept in water for 3 h, at 28?¡À1 degree C, served as control (Seedling).
Contributor(s) Tyagi AK,Kapoor S,Khurana JP,Ray S
Citation(s) 17293439,19490110
 
Submission date 29-Jan-07
Contact name Akhilesh K. Tyagi
E-mail(s) akhilesh@genomeindia.org
Phone 91-11-24113216
Fax 91-11-24119430
URL
Organization name University of Delhi South Campus
Department Department of Plant Molecular Biology
Lab Plant Genomics and Molecular Biology
Street address Benito Juarez Road
City New Delhi
State/province Delhi
ZIP/Postal code 110 021
Country India

  ID Platform Sample Name
1. GSM159259 Affymetrix 7-day-old Seedling, biological rep 1
2. GSM159260 Affymetrix 7-day-old Seedling, biological rep 2
3. GSM159261 Affymetrix 7-day-old Seedling, biological rep 3
4. GSM159262 Affymetrix Drought stress, biological rep 1
5. GSM159263 Affymetrix Drought stress, biological rep 2
6. GSM159264 Affymetrix Drought stress, biological rep 3
7. GSM159265 Affymetrix Salt stress, biological rep 1
8. GSM159266 Affymetrix Salt stress, biological rep 2
9. GSM159267 Affymetrix Salt stress, biological rep 3
10. GSM159268 Affymetrix Cold stress, biological rep 1
11. GSM159269 Affymetrix Cold stress, biological rep 2
12. GSM159270 Affymetrix Cold stress, biological rep 3