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Experiment ID
GSE6901
Experiment Title
Expression data for stress treatment in rice seedlings
Number of Samples
12
Organism(s)
Oryza sativa
Summary
Drought, salinity and sub-optimal temperatures are stresses that cause adverse effects on the growth of plants and the productivity of crops. In this study, we have analyzed the expression profiles of rice genes under control and abiotic stress conditions using microarray technology to identify the genes differentially expressed during various abiotic stresses.
Keywords: Stress treatment
Overall design
Seven-day-old light-grown rice seedlings grown under controlled conditions and those subjected to various abiotic stress conditions were used for RNA extraction and hybridization on Affymetrix microarrays. Three biological replicates of each sample were used for microarray analysis. For salt treatment (SS), the rice seedlings were transferred to a beaker containing 200 mM NaCl solution for 3 h. For desiccation (DS), rice seedlings were dried for 3 h between folds of tissue paper at 28?¡À1 degree C, in a culture room. For cold treatment (CS), the seedlings were kept at 4?¡À1 degree C for 3 h. The seedlings kept in water for 3 h, at 28?¡À1 degree C, served as control (Seedling).
Contributor(s)
Tyagi AK,Kapoor S,Khurana JP,Ray S
Citation(s)
17293439
,
19490110
Submission date
29-Jan-07
Contact name
Akhilesh K. Tyagi
E-mail(s)
akhilesh@genomeindia.org
Phone
91-11-24113216
Fax
91-11-24119430
URL
Organization name
University of Delhi South Campus
Department
Department of Plant Molecular Biology
Lab
Plant Genomics and Molecular Biology
Street address
Benito Juarez Road
City
New Delhi
State/province
Delhi
ZIP/Postal code
110 021
Country
India
ID
Platform
Sample Name
1.
GSM159259
Affymetrix
7-day-old Seedling, biological rep 1
2.
GSM159260
Affymetrix
7-day-old Seedling, biological rep 2
3.
GSM159261
Affymetrix
7-day-old Seedling, biological rep 3
4.
GSM159262
Affymetrix
Drought stress, biological rep 1
5.
GSM159263
Affymetrix
Drought stress, biological rep 2
6.
GSM159264
Affymetrix
Drought stress, biological rep 3
7.
GSM159265
Affymetrix
Salt stress, biological rep 1
8.
GSM159266
Affymetrix
Salt stress, biological rep 2
9.
GSM159267
Affymetrix
Salt stress, biological rep 3
10.
GSM159268
Affymetrix
Cold stress, biological rep 1
11.
GSM159269
Affymetrix
Cold stress, biological rep 2
12.
GSM159270
Affymetrix
Cold stress, biological rep 3
Available Data:
Platforms: 5
Experiments: 68
Hybridations: 1378