Rice Array Element Search


To determine which probes from each platform mapped to a specific target, each probe was searched against a successive series of nucleotide/protein databases to find if that particular oligo mapped to any targets at 100% coverage and 100% identity. If there was a match, that probe was considered to be mapped and removed as a list of candidates to be searched against in the following round of database searches.

The databases used were as follows:
  1. MSU/TIGR Rice Genome Annotation v6 cDNAs
  2. RAP2 Rice Genome Annotation cDNAs
  3. Rice KOME full-length cDNAs
  4. RAP3 Rice Genome Annotation cDNAs

Alignment searches were conducted using NCBI BLAST. In the case of the Affymetrix searches, a probe set was considered to have been mapped if more than half of the entire set of designed probes aligned with 100% identity and coverage.

Download the whole rice array elements information (zip, 6M) (Updated February 25, 2010).

The data types in the Rice Array Element Search are the following:

Array Element ID: a unique identifier generated by manufacture to reference this probe.

Array Platform: The array platform containing this probe: Affymetrix, Agilent, Yale/BGI, NSF 20K and NSF 45K (23K A or 23K B).

Matched Gene ID: The sequence this probe aligns to at 100% length and identity. Example match IDs include MSU/TIGR Rice Genome Annotation release 6 models (e.g. LOC_Os01g01150.1), RAP2 (e.g. Os01g0113200) and full length cDNAs (e.g. AF044260).

Probe Sequence: The sequence of the probe.



Last modified: Monday, 07-Jun-2010 17:20:41 Pacific Daylight Time